PSFC是什么

Veblen

请问一下大家Phase-sensitive flow cytometry是什么？它与普通流式的区别在哪里呢？

Veblen

大家有了解吗 请问

Veblen

能不能简单的解释一下呢

niwanmao

Veblen 发表于 2017-12-29 18:52
能不能简单的解释一下呢

大概看了下，感觉应该是体现一种功能指标动态变化的改良性技术：
Phase-sensitive flow cytometry (PSFC) is a technique in which fluorescence excited state decay times are measured as fluorescently labeled cells rapidly transit a finely focused, frequency-modulated laser beam. With PSFC the fluorescence lifetime is taken as a cytometric parameter to differentiate intracellular events that are challenging to distinguish with standard flow cytometry. For example PSFC can report changes in protein conformation, expression, interactions, and movement, as well as differences in intracellular microenvironments.

摘自Nature文章：https://www.nature.com/articles/srep40341