倪老师,我找到了两篇文献,是一个流式专家的blog上看到的,总结了isotype的缺陷什么的,给大家贴上哈:
1. Hulspas R, O’Gorman MRG, Wood BL, Gratama JW, Sutherland DR. Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry Part B 2009; 76B: 355–364.
2. 第二篇给大家提供个地址,DOI: 10.1002/cyto.a.20333