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发表于 2012-4-13 10:08:53
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zark_00 发表于 2012-4-13 08:34
就是简称DIO的一种绿荧光染料，能与细胞膜结合，能标记很多种细胞。
哦。学习了。
从文献来看，你的浓度应该是偏高了：
The DIOC18 staining, which turned out to be far superior and which was therefore used in all subsequent experiments, was conducted as follows: a stock solution was prepared by dissolving DIOC18 (Sigma) in DMSO (2 mg/ml; Sigma) over
night with agitation. The NK cells (106 cells/ml) were incubated in 10 ug/ml DIOC18 (final concentration)
for 1 h at 37℃. Longer incubation periods as employed elsewhere (Kroesen et al., 1992) were not necessary to obtain ample staining. Cells were washed twice and maintained in medium (RPMI 1640 [Bio Whittaker, Boehringer Ingelheim, Germany] supplemented with 10% bovine serum and L-glutamine).
全文见：
100224TO7.pdf
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发表于 2012-4-12 10:18:34
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