老师们好，我目前实验是尝试用线粒体示踪剂（MitoTracker 货号: M7510）进行红细胞的染色，参考的是一篇文章中下列的做法For mitochondrial content in mature RBCs, PBMCs were removed from peripheral blood by density centrifugation using Lymphoprep™ (Stem Cell Technologies). The cell pellet was washed four times (3000 rpm, 8 min, 4°C) with PBS supplemented with 2 mM EDTA and 10 mM Glucose. The cell pellet was then re-suspended in PBS supplemented with 10 mM Glucose and MitoTracker Deep Red (MTDR, 200 nM; Thermo-Fisher) and incubated for 30 min at 37°C)。但是每次染完细胞都没有明显的分群，只有整体荧光强度略微的提高，难道是我的红细胞中真的都没有线粒体吗？附上原始文件，望各路大神解惑！
发表于 2022-9-27 20:24:34
For mitochondrial content in mature RBCs, PBMCs were removed from peripheral blood by density centrifugation using Lymphoprep™ (Stem Cell Technologies). The cell pellet was washed four times (3000 rpm, 8 min, 4°C) with PBS supplemented with 2 mM EDTA and 10 mM Glucose. The cell pellet was then re-suspended in PBS supplemented with 10 mM Glucose and MitoTracker Deep Red (MTDR, 200 nM; Thermo-Fisher) and incubated for 30 min at 37°C)。但是每次染完细胞都没有明显的分群，只有整体荧光强度略微的提高，难道是我的红细胞中真的都没有线粒体吗？附上原始文件，望各路大神解惑！
发表于 2022-9-27 21:08:04
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