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发表于 2022-4-2 18:43:39
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首先，就是在美天旎、stemcell用于小鼠髓系细胞分选产品和资料中，都提及这点以及有相关产品整合在这个解决方案里，这个我之前用过。在流式检测样本制备的材料中也是这样。做TBNK 不用消化，髓系细胞需要。
另外你要文章，我就去查了一篇 可以看下。
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364618/
Identification and isolation of splenic tissue-resident macrophage sub-populations by flow cytometry
Abstract
Tissue-resident macrophages in the spleen, including red pulp and white pulp macrophages, marginal zone macrophages (MZMs) and marginal zone metallophilic macrophages (MMMs), are highly heterogeneous as a consequence of adaptation to tissue-specific environments. Each macrophage sub-population in the spleen is usually identified based on the localization, morphology and membrane antigen expression by immunohistochemistry. However, their phenotypical and functional characteristics remain incompletely understood due to the difficulty of identification and isolation by flow cytometry. We used a cocktail of three enzymes (Collagenase D, Dispase I and DNase I), rather than traditional mechanical grinding, for isolation of each sub-population, which resulted in significant improvement of isolation of these macrophage sub-populations, particularly MZMs and MMMs, as determined by CD11bhiF4/80medTim4hi and CD11bhiF4/80medTim4med, respectively. This method should be helpful for molecular and functional characterization of each splenic resident macrophage sub-population. |
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