1. 图1中的S1, S2, S3 就是图2中的 P1, P2,P3吗？
按图2文献的说法，“ The CD11b+ cells found in the LP of the small intestine comprised CX3CR1int and CX3CR1hi cells (Fig. 2A). In contrast tothe CX3CR1hi cells that are only constituted of Ms (Fig. 2B),the CX3CR1int cells were heterogeneous and contained CD11b+-type Int-DCs that can be readily gated out on the basis of theirCD11chiCD64− phenotype (Fig. 2C). Analysis of the remaining,Int-DC-deprived, CX3CR1int cells on a Ly-6C-MHCII plot led tothe identification of three populations (Fig. 2C). Population 1(P1) resembled Ly-6Chi blood monocytes in terms of FSC-SSCprofile (data not shown) and of its Ly-6Chi, CD64low, MHCII−,CD11c− to low, CX3CR1int, and CD11b+ phenotype (Fig. 2C and D).P1 cells in the LP can also be identified on the basis of their uniqueCD11b+Ly-6Chi phenotype (Fig. 2E). Population 2 (P2) was composed of Ly-6Cint to hiMHCII+ cells whereas population 3 (P3) hada Ly-6ClowMHCII+ phenotype identical to that of the CX3CR1highMs that are denoted as P4 cells (Fig. 2B and C). ”
可父级门不是一样的呀？ 昨天问了一位老师，他说：
The best way to distinguish Ly6Chi monocyte/macrophage and Ly6Clo macrophage (not including monocyte) is to first gate CD11c-CD64+ and then gate with Ly6C and MHC-II.
发表于 2022-5-20 15:33:01
发表于 2022-5-21 09:01:32
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