多参数积分系统鉴别伯基特淋巴瘤和CD10+弥漫大B细胞淋巴瘤

niwanmao

Am J Clin Pathol. 2012 Apr;137(4):665-70.

Differentiating between Burkitt lymphoma and CD10+ diffuse large B-cell lymphoma: the role of commonly used flow cytometry cell markers and the application of a multiparameter scoring system.
McGowan P   , Nelles N   , Wimmer J   , Williams D   , Wen J   , Li M   , Ewton A   , Curry C   , Zu Y   , Sheehan A   , Chang CC   .
Source
Pathology and Laboratory Medicine, The Methodist Hospital, Houston, TX, USA.

Abstract
The goal of this study was to evaluate routine flow cytometric (FC) immunophenotypic markers in differentiating between Burkitt lymphoma (BL) and CD10+ diffuse large B-cell lymphoma (DLBCL). We performed retrospective analysis of FC data from 55 patients. We evaluated 9 FC parameters: forward and side scatter (FSC and SSC); mean fluorescent intensity (MFI) for CD20, CD10, CD38, CD79b, CD43, and CD71; and the percentage of neoplastic cells positive for CD71 (%CD71). The FSC; MFIs of CD10, CD43, CD79b, and CD71; and %CD71 cells were significantly different between BL and CD10+ DLBCL (P < .05; Student t test). A 5-point scoring system (FSC, %CD71, and MFIs of CD43, CD79b, and CD71) was devised, and 6 (60%) of 10 BLs scored 3 or greater and 1 (10%) of 10 CD10+ DLBCLs scored 3 (P = .04; χ(2)). Our findings indicate that routine FC parameters can aid in differentiating BL from CD10+ DLBCL.
PMID: 22431545 [PubMed - indexed for MEDLINE]

全文见本帖第3楼，在线阅读免流星。

niwanmao

北京大学人民医院的刘艳荣教授对此文的评述如下：

评论1

评论2

niwanmao

全文PDF附件见： BL_DLBCL.pdf (361.59 KB, 下载次数: 7, 售价: 2 流星)

2012-7-30 20:50 上传

点击文件名下载附件

，下载需要2颗流星（<a href="http://www.flowcyto.cn/bbs/forum.php?mod=viewthread&amp;tid=1520" target="_blank">为什么？</a>）
<br>
使用流式中文网资料阅读器在线阅读，免流星，内容完全一样（<b>IE8和360浏览器无法显示，建议使用IE9和google chrome浏览器。</b><a href="http://www.flowcyto.cn/bbs/forum.php?mod=viewthread&amp;tid=1527" target="_blank">使用说明</a>）
<br>
<script type="text/javascript" src="flash/flowcytoview/swfobject.js"></script>
   <script type="text/javascript">
                        function thisMovie(movieName) {
                         if (navigator.appName.indexOf("Microsoft") != -1) {
                                 return window[movieName];
                         } else {
                                 return document[movieName];
                         }
                         }

                        function SaveState(){
                        thisMovie("flowcytoView").SaveState();
                        }

                        window.onbeforeunload =   function()
                        { SaveState();}

            var swfVersionStr = "10.0.0";
            var xiSwfUrlStr = "playerProductInstall.swf";
            var flashvars = {Swffile:"flash/2012/3q/BL_DLBCL/{BL_DLBCL[*,0].swf,7}"};
            var params = {};
            params.quality = "high";
            params.bgcolor = "#869ca7";
            params.allowscriptaccess = "sameDomain";
            params.allowfullscreen = "true";
            var attributes = {};
            attributes.id = "flowcytoView";
            attributes.name = "flowcytoView";
            attributes.align = "middle";
            swfobject.embedSWF(
                "flash/flowcytoview/flowcytoView.swf", "flashContent",
                "750", "600",
                swfVersionStr, xiSwfUrlStr,
                flashvars, params, attributes);
            swfobject.createCSS("#flashContent", "display:block;text-align:left;");
</script>

<div id="flashContent">
      <p>要正常查看本资料，需要安装Adobe Flash Player插件（版本号为10.0以上）</p>
       <script type="text/javascript">
                var pageHost = ((document.location.protocol == "https:") ? "https://" :        "http://");
                document.write("<a href='http://www.adobe.com/go/getflashplayer'><img src='" + pageHost + "www.adobe.com/images/shared/download_buttons/get_flash_player.gif' alt='获取 Adobe Flash player' /></a>" );
        </script>
</div>