流式杂谈和进展

流式细胞术可增加肺泡灌洗液中肿瘤细胞检测灵敏度
  • 楼主 huzengwu
  • 247932012-7-29 09:36:52
全文地址  http://onlinelibrary.wiley.com/doi/10.1002/cyto.b.21033/pdf
Flow cytometry increases the sensitivity of detection of leukemia and lymphoma cells in bronchoalveolar lavage specimens
Abstract
BACKGROUND:
Recent studies have definitively determined that flow cytometry (FC) is significantly more sensitive than cytomorphology (CM) in detection of hematolymphoid neoplasms (HLNs). However, its utility in paucicellular bronchoalveolar lavage (BAL) specimens has not been established.

METHODS:
FC was performed on BAL specimens submitted from 44 patients with a prior diagnosis of HLN. Panels chosen were based upon cellularity of specimen and patient history. FC results were compared with concurrent CM evaluations.

RESULTS:
All 44 BALs were deemed satisfactory for FC and yielded informative results that assisted in diagnosis. Diagnoses included 22/44 B-cell neoplasms, 16/44 T-cell neoplasms, four/44 myeloid neoplasms, and two/44 plasma cell neoplasms. Overall concordance was demonstrated between FC and CM in 77% (34/44) of cases. In nine/44 cases (20%), one technique (FC or CM) clearly detected malignant cells when the other did not. FC was more sensitive than CM in detecting a HLN in eight/nine discordant cases. In only one case (one/44, 2%) were malignant HLN cells suspected by CM, but not identified by FC (one/44, 2%).

CONCLUSION:
We demonstrate, in the largest series published to date, that FC can be performed on BAL specimens. FC is indicated in evaluation of BAL for HLN and improves sensitivity of detection of HLN over CM alone. An integrated FC and CM approach is superior to either technique alone in diagnostic evaluation of BAL. Published 2012 Wiley Periodicals, Inc.
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老师,能下到全文就好了

这篇好新啊,7月26日才刚刚出来的,连全文链接都没有呢。
看了摘要,这篇文章其实也就是说明了流式在细胞少的体液检测中的应用,这里用的是肺泡灌洗液,我们平时胸水、腹水、脑脊液等都做过,确实很有用处,检测用的panel就是根据他们白血病或淋巴瘤初发时的特异性表型决定的。说非常有特色也说不上吧。
文献代理确实有点难找,平时只能在鸭绿江或者丁香园两个地方求助文献,自己想找点代理,难!
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