2010年8月1日前归档

9921 niwanmao 管理组

标题:关于树突状细胞表面标志物

今天帮人做实验,用CD80、CD83、CD86看树突状细胞激活情况,这倒好分析,但如何圈出树突状细胞就存疑问了,她说她的细胞是经梯度密度离心找出PBMC的,又帖壁吸附了,只剩树突状细胞了,但我看前向侧向角好像还有不少的淋巴及中性粒,。印象中CD14+算单核的标志物,查了文献有说那树突的标志是CD11c+CD1a+CD14-,y有点晕,到底哪些标志物是特异的还经刺激培养后又不会有太大的变化,期待中。。。。。。

回答者:niwanmao

给你转一段文字,不翻译了,直接看英文更好理解。对你了解树突状细胞非常有帮助:

Dendritic cells (DCs) are immune cells and form part of the mammalian immune system. Their main function is to process antigen material and present it on their surface to other cells of the immune system. Dendritic cells are present in small quantities in tissues that are in contact with the external environment, mainly the skin (where they are often called Langerhans cells) and the inner lining of the nose, lung, stomach and intestines. They can also be found at an immature state in the blood. Once activated, they migrate to the lymphoid tissues where they interact with T cells and B cells to initiate and shape the immune response. At certain development stages they grow branched projections, the dendrites, that give the cell its name. However, these do not have any special relation with neurons, which also possess similar appendages. Immature dendritic cells are also called veiled cells, in which case they possess large cytoplasmic \'veils\' rather than dendrites.

下面是一些树突状细胞的相关标记及其表达意义:

ADAM19 (MADDAM)

  • a molecular marker for human dendritic cells and not expressed in macrophages. PMID: 15896713

BDCA-2

  • a cell surface protein whose expression is restricted to human plasmacytoid dendritic cells, in a differentiation stage-specific manner. PMID: 16998465

CD1a

  • a marker of immature DCs, plays a role in tumorigenesis. PMID: 16159438

  • a human epidermal dendritic cell marker related to class I MHC molecules. PMID: 2784820

CD11c

CD21

CD83

CD86

  • a specific marker for canine monocyte-derived dendritic cells. PMID: 16202456

CD208

  • CD208 DC lysosomal-associated protein is a marker of activated human dendritic cells.PMID: 16899762

  • Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, a member of the lysosomal associated membrane protein (LAMP) family, is specifically expressed by human DCs on activation. PMID: 14982840

CLIP-170/restin

Clusterin

DC-LAMP (CD208)

  • a member of the lysosomal-associated membrane protein (LAMP) family, is specifically expressed by human dendritic cells (DC) upon activation and therefore serves as marker of human DC maturation. PMID: 12938238PMID: 14982840

DEC-205

  • a good marker of the lymphoid-related regulatory type of dendritic cell (DC). PMID: 10784619

Estrogen Receptor-alpha

  • a novel marker expressed by follicular dendritic cells in lymph nodes and tumor-associated lymphoid infiltrates. PMID: 14507640

Fascin

  • a 55-kd actin-bundling protein, represents a highly selective marker for dendritic cells of lymphoid tissues and peripheral blood. PMID: 12219775

HLA-DR

  • HLA-DR antigen is present on the plasma membrane and within some of the dilated cisternae. PMID: 3515706

MADDAM (metalloprotease and disintegrin dendritic antigen marker)

  • an important marker for the differentiation and characterization of DCs and the distinction between MACs and DCs. PMID: 10887142

NLDC-145

  • a dendritic cell specific marker NLDC. PMID: 3265929

  • recognizes DEC-205, a 205-kDa integral membrane glycoprotein. PMID: 7758125PMID: 7553896

  • NLDC-145 detects an antigen (DEC-205) expressed at high levels by mouse dendritic cells (DCs) and thymic epithelial cells. PMID: 7553896

S-100

  • an immunohistochemical marker for a subset of dendritic cells, the interdigitating reticulum cells (IDRCs), which are mainly located in T-dependent areas of lymphoid tissues. PMID: 2878529

2009年8月12日 0:54

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有 7 条回复

2楼 摩登萝卜 LV2

前辈,有没有像这样关于巨噬细胞的这么详细的资料啊?谢谢

3楼 crxdw LV2

您好,请问有没有猪的DC这么详细的资料啊?

4楼 niwanmao 楼主


猪树突状细胞Developmental and Comparative Immunology 2009年有一篇,不知道你看过没有?
《The porcine dendritic cell family》

2013-06-24_142635.gif  (2013-6-24 14:27 上传)

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The porcine dendritic cell family.pdf  (2013-6-24 14:28 上传)

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5楼 crxdw LV2

谢谢您了,前辈!不知道您能否帮我找到猪肥大细胞的标志物

6楼 crxdw LV2

求助了前辈,猪肥大细胞的有吗?谢谢您了

7楼 niwanmao 楼主

引用: crxdw 发表于 2013-6-26 09:36
求助了前辈,猪肥大细胞的有吗?谢谢您了

看看这篇是否对你有帮助:http://onlinelibrary.wiley.com/d ... 9779614568FE.d04t02

8楼 xiaopi118 LV2

学习了,谢谢倪老师