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微液流图像细胞术（Microfluidic image cytometry，μFIC）是一种利用微米级管路，以可控性的化学环境培养和处理细胞，同时可进行细胞毒性检测的新型方法。本文作者在文中开发、评估了基于荧光的μFIC，并将其应用于高通量细胞周期分析。该微液流装置配备了浓度梯度产生器和8个细胞培养管道，用于检测紫杉醇诱导的HeLa细胞细胞周期变化。
全文链接：http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22261/pdf 如果哪位站友有权限下载，欢迎分享学习。
可能大多数站友都是第一次接触μFIC，我也是，故从网上找了张图片，了解一下这种新型的细胞培养和检测方法。
双电极μFIC
The dielectrophoretic (DEP)-microfluidic device and the cell patterning process. (A) The DEP-microfluidic device. The PDMS chip with channel network (shown in blue) is bonded to glass substrate with ITO electrodes (shown in close-up pictures, scale bar=100 m). The ITO connection bars leading to the electrodes are outlined. (B) Femlab simulation of the electric field in the space separating the electrodes, showing convergence of the electric field at the electrode tips, the property used for cell capture. (C) Cell patterning process by positive DEP force (see Supplementary Movie 1). (D) A partial view of four columns of patterned cells from each of the four channels in (A). (E) Patterned HUVEC–HUVEC pair, HUVEC–A549 pair and a single HUVEC cell in the device after 4 h of culturing in the complete medium. The A549 cell is manually outlined.
Cytometry A. 2013 Feb 15. doi: 10.1002/cyto.a.22261. [Epub ahead of print]
High throughput cell cycle analysis using microfluidic image cytometry (μFIC).
Yoo HJ , Park J , Yoon TH .
Source
Department of Chemistry, Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea.
Abstract
Microfluidic image cytometry (μFIC) is a novel approach for the cytotoxicity assessment of the cells cultured and treated within microfluidic channels under a precisely controlled chemical environment. Here, following our previous morphology-, and MTT absorbance-based μFIC, we are presenting our recent effort to develop, evaluate, and apply a high throughput cell cycle analysis method using fluorescence-based μFIC. A microfluidic device with a concentrantion gradient generator (CGG) and eight straight cell culture channels was fabricated, optimized, and applied for the assessment of paclitaxel-induced cell cycle changes of HeLa cells. Throughout this study, we have shown that the cell cycle analysis using fluorescence-based μFIC was able to provide comparable experimental data with those of flow cytometry. Moreover, cell cycle analysis using μFIC can also provide further advantages over flow cytometry, such as higher throughput, lower assay cost, less generation of toxic waste, and etc., which should have significant implications in pharmaceutical and biological applications as a future high throughput cell cycle analysis platform.
PMID: 23418122
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发表于 2013-2-20 13:32:56
发表于 2013-2-23 15:31:28
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