微液流图像细胞术对hPSC单个细胞定量分析

niwanmao

昨日发了一篇《利用微液流图像细胞术（μFIC）高通量分析细胞周期》，今天再发一篇微液流图像细胞术（μFIC）在人多能干细胞中的研究，希望对于研究多能干细胞的朋友会有所帮助。
这一篇就不翻译了，因为我对此也是不太了解，有兴趣的朋友可在线免费阅读全文了解一下。

Fig. 1 (a) The extrinsic factors such as soluble growth factors, cell-cell interactions, and ECM play an important role in controlling stem cell fate in the microenvironment. (b) Schematic illustration of a microfluidic hPSC array for hPSC culture and phenotype assay. (c) Microfluidic image cytometry (MIC) was conducted followed by segmentation and quantification analysis.

μFIC培养多能干细胞流程图

Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions
Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL^-1 of bFGF on 20 mg mL^-1 of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitativeMIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.

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