BD公司的CantoII使用的关机液可以用什么代替？

lejiang

如题，因为听说关机液似乎就是纯水，不过不能确定，如果真的是纯水的话，我们实验室自己的纯水机就可以造出来，所以想问问了解情况的朋友，是不是可以用纯水代替。

niwanmao

我没用过CANTO II ，但是从下面这个来自http://pathology.jhu.edu/flow/COG%20procedures/Canto_Shutdown_7-20-10.pdf的关机步骤来看，似乎没有涉及到关机液这一说法啊。不知道这一说法从何而来？
从这个关机步骤来看，和CALIBUR的关机步骤基本上差不多，就是先用漂白水冲，再用蒸馏水冲，最后关机。

FACSCanto Shutdown Procedure
1.Double click to open FACS Diva Software.
For Canto A, enter password “pog”.
For Canto II, there is no password.
2.Once in FACS Diva Software, click the “+”(plus) sign next to “DAILY QC” folder.
3.Double click on “Cleaning tube” to open the experiment.
4.For Canto A, make sure the sample probe is in the “UP” position.
For Canto II, there is only one position so no need to adjust.
5.Click the green arrow ONCE next to Tube 1.
6.Place a tube of cleaning solution (i.e. 1:20 Bleach, Contrad, or Coulter Clens) on the Canto and select ‘Acquire Data’. Do Not select ‘Record’! Acquire for 5 minutes.
Note: Bleach tube should be changed at least once a week.
7.For Canto A: Click on ‘Stop Acquiring’ when finished. A message will appear that the computer is “Processing Data”. When “Processing Data” is finished in a few seconds, remove tube by clicking the “Remove Tube” button on the screen and then swing the tube platform arm over and remove tube from the sample probe.
For Canto II: Click on “Stop Acquiring” when finished. The tube platform will automatically come down. Remove tube right away. There is no “Remove Tube” button on Canto II.
8.Repeat steps 5-7 using a deionized water tube for 5 minutes (run water for the same amount of time that you run the cleaning solution).
9.Under ‘Cytometer’ along the menu at the top of page, select ‘Fluidics Shutdown’. A comment will appear:
For Canto A ‘Fluidics shutdown can take up to 7 min’.
For Canto II ‘Fluidics shutdown can take up to 5 min’ .
Click ‘OK’.
10.When Fluidics Shutdown is complete, a message will appear;
“Fluidics Shutdown is complete. It is now safe to turn off the instrument.”
11.Click OK
12.Under ‘File’ at the top of the page, select ‘Quit’. Be sure ‘Exit application only’ is selected, then click ‘OK’.
13.After all functions on the desktop are closed, shut down the computer. Click START at the bottom left corner of the page.
Select ‘Shutdown’ and when the comment “What do you want your computer to do?” appears, be sure the comment in the box is ‘Shutdown”. Click ‘OK’.
14.Turn off the instrument by pressing the green button on the left side of the outside of the FACSCanto.
15.Record the shutdown steps, your initials, and time shutdown in the QC and Maintenance Book.

chujindong

回复 niwanmao 的帖子

这个是好东西啊！我们用的就是FACSCanto，我们的关机液用的是去离子水。关机液的桶上明明白白的写着"DI water".
这里写的cleaning solution就是漂白粉是吧？

niwanmao

chujindong 发表于 2011-6-21 23:14

                               
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回复 niwanmao 的帖子

这个是好东西啊！我们用的就是FACSCanto，我们的关机液用的是去离子水。关机液的桶 ...

是的。冲洗是用蒸馏水，管子里面先用漂白水，后用蒸馏水。

可可爸

BD CantoII 有 关机液  我们就是用的去离子水。

aninawang

shutdown solution 可以用纯水代替，cleaning solution 可以用5%活性成分的次氯酸代替。

luxj807

全部都可以用去离子水代替，哥就是这么做的。
当然这是BD技术支持说可以。

lejiang

各位，多谢多谢

niwanmao

今年买了CANTO II之后，才知道了shutdown solution和cleaning solution，从原理来看，应该是可以用蒸馏水和次氯酸钠溶液代替的。

lejiang

谢谢倪老师